FIG 5.

Single-cycle infectivity of the replication-competent isolates from the reservoirs of patients. The infectivity of each viral isolate was assessed by a single-cycle infection assay in TZM-bl cells. These cells express CD4 and both viral coreceptors, as well as the β-galactosidase reporter gene under the control of HIV LTR. The cells were exposed to serial dilutions of viral isolates for 24 h and then lysed, and the production of β-galactosidase was estimated by a chemiluminescent assay (Roche). Infectivity was measured as a function of p24 input and expressed as the percentage of the value for the most infectious isolate for each patient. The means and standard deviations from at least three independent experiments are shown. Geometric symbols indicate isolates for which identical sequences were identified in the reservoir. The reference strain NL-AD8 (gray bars) was used as a control.