FIG 1.

Ciprofloxacin-mediated stimulation of pyocin expression in ΔxerC strains is RecA independent. (A) Representative growth curves (OD₆₀₀) and luminescence traces (P₀₇₉₉₀-lux) of wild-type PA14 (MTC2280) and ΔrecA (MTC2302) strains treated (gray) or not (black) with 0.03 μg/mL ciprofloxacin (Cipro). Note that the y axis scales on the luminescence graphs vary. Light gray shading surrounding the traces indicates standard deviation from three technical replicates. Time is indicated in hours. (B) Representative growth curves and luminescence traces as in panel A, but for ΔxerC (MTC2297) and ΔxerC ΔrecA (MTC2301) strains. (C and D) OD-normalized luminescence traces of the indicated strains from panels A and B, respectively.