FIG 3.
Mitomycin C-mediated stimulation of pyocin expression in wild-type and ΔxerC strains requires PrtN. (A) Representative growth curves (OD600) and luminescence traces (P07990-lux) of wild-type PA14 (MTC2280) and ΔprtN (MTC2303) strains treated (gray) or not (black) with 0.1 μg/mL mitomycin C (MMC). Note that the y axis scales on the luminescence graphs vary. Light gray shading surrounding the traces indicates standard deviation from three technical replicates. Time is indicated in hours. (B) Representative growth curves and luminescence traces as in panel A, but for ΔxerC (MTC2297) and ΔxerC ΔprtN (MTC2298) strains. (C and D) OD-normalized luminescence traces of the indicated strains from panels A and B, respectively.