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. 2022 Jul 22;7(14):e158523. doi: 10.1172/jci.insight.158523

Figure 2. RIPC induces differential transcriptomic reprogramming in murine neutrophils and renal tubular epithelial cells.

Figure 2

After induction application of RIPC or control procedure, IRI was induced in WT mice by clamping of the renal pedicles for 32 minutes. Twenty-four hours after the surgery, mice were sacrificed. In order to conduct RNA-Seq, neutrophils and proximal renal tubular epithelial cells (PTECs) were isolated 24 hours after the IRI procedure (n = 3 biological replicate libraries per group). (A–H) Heatmaps with hierarchical tree and volcano plots comparing differences of RNA-Seq–based gene expression values in PMNs from sham mice (A and B) and IRI mice (E and F), as well as from PTECs from sham mice (C and D) and IRI mice (G and H). Biostatistical analysis described in Methods; *P <0.05.