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. 2022 Jul 22;7(14):e158523. doi: 10.1172/jci.insight.158523

Figure 8. Sema5b regulates RIPC-induced renal protection from IRI in vivo.

Figure 8

After induction of general anesthesia, renal IRI was induced in WT and Sema5b-deficient mice by clamping of the renal pedicles for 32 minutes. Some mice received a blocking Sema5b antibody (50 μg/mouse) or were subjected to RIPCs before IRI induction. Twenty-four hours after the surgery, mice were sacrificed. (A) The recruitment of neutrophils (PMNs) into the kidney was analyzed by flow cytometry (n = 4). (B) Serum creatinine levels were measured by a photometric assay (n = 4). (C) The biomarkers TIMP-2 and IGFBP7 were measured in urine samples 24 hours after renal IRI (n = 4). (D) Quantification of histological tubular injury (n = 4). (E) Detection of injected Sema5b antibody in the plasma and urine by sandwich ELISA (n = 6). (F) Urinary levels of Sema5b in cardiac surgery patients from the RIPCrenal trial before and 4 hours after RIPC application (n = 20/group). One-way ANOVA followed by Bonferroni testing was used for statistical analysis; *P < 0.05.