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. 2022 Jul 22;7(14):e158107. doi: 10.1172/jci.insight.158107

Figure 1. Generation of genomically encoded annexin A6GFP using CRISPR/Cas9 genome editing.

Figure 1

(A) Targeting strategy for generating genomically encoded annexin A6GFP at the endogenous annexin A6 locus. Red lettering indicates protospacer adjacent motif sequence. Lowercase lettering indicates synonymous mutations in targeted allele. (B) Anxa6gfp mouse generation strategy. Genotyping schematic and PCR screening of Anxa6gfp of 6 heterozygous N1 offspring lines. (C) Representative sequence chromatograms of in-frame GFP insertion into the annexin A6 locus in Anxa6gfp CRISPR/Cas9-edited mouse line 46.