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. 2022 Jul 22;7(14):e158107. doi: 10.1172/jci.insight.158107

Figure 6. Recombinant annexin A6 associates with injured myoblasts and myofibers.

Figure 6

(A) Rat L6 myoblasts were injured with LLO and then incubated with recombinant annexin A6 conjugated to 488 (rA6-488). The percentage of rA6-488–positive cells increased with increasing concentrations of annexin protein. (B) Total fluorescence intensity of rA6-488–positive cells increased with increasing concentrations of rA6-488, normalized to 1.0 μg/mL. (C) Increasing rA6-488 incubation time increased fluorescence signal of injured cells but not noninjured control cells. (D) Incubation of myofibers in recombinant annexin A6 (rANXA6) for either 5 or 60 minutes both reduced FM 4-64 dye uptake after injury compared with BSA-treated control myofibers. Scale bar: 5 μm. n ≥ 4 cell platings. n ≥ 6 myofibers from n = 4 isolations. *P < 0.05 by 1-way ANOVA.