TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Descriptiona | Reference or source |
|---|---|---|
| Escherichia coli | ||
| JM109 | recA1 supE44 hsdR17 gyrA96 relA1 thi (lac-proAB) (F′ traD36 proAB+lacIqlac M15) | Promega |
| NFR502 | thr-1 ara-14 leuB6 DE(gpt-proA)62 lacY1 tsx-33 nfsB-2 qsr′-0 glnV44 galK2 LAM-nfsA1 Rac-0 hisG4 rfbD1 rpsL31(Smr) kdgK51 xylA5 mtl-1 argE3 thi-1 | 21; CGSC6838 |
| Pseudomonas putida | ||
| HS12 | NB+, wild-type, pNB1 and pNB2 carrier | |
| HS121 | NB−, a spontaneous mutant of HS12, pNB2 carrier | This study |
| HS124 | NB−, a cured mutant of HS12, pNB1 carrier | This study |
| Plasmids | ||
| pNB1 | 59.1-kb NB catabolic plasmid of P. putida HS12 | This study |
| pNB2 | 43.8-kb NB catabolic plasmid of P. putida HS12 | This study |
| pACYC184 | Cmr Tcr | New England Biolabs |
| pBR328 | Apr Cmr Tcr | New England Biolabs |
| pBluescript SK(+) | Apr | Stratagene |
| pBluescript KS(+) | Apr | Stratagene |
| pHS289 | pBluescript KS carrying a 1.1-kb SmaI-SnaBI fragment from pNB1 | This study |
| pHS291 | pBR328 carrying a 6.6-kb SnaBI-SmaI fragment from pNB1 | This study |
| pHS2910 | pBR328 carrying a 2.3-kb SnaBI-AsnI fragment from pNB1 | This study |
| pHS2911 | pBluescript SK carrying a 4.8-kb SnaBI-PstI fragment from pNB1 | This study |
| pHS2912 | pBluescript KS carrying a 2.4-kb EcoRI-PstI fragment from pNB1 | This study |
| pHS2916 | pBluescript SK carrying a 1.8-kb PstI fragment from pNB1 | This study |
| pHS35 | pACYC184 carrying a 9.0-kb EcoRI fragment with nbzB from pNB2 | This study |
| pHS3951 | pBR328 carrying a 1.1-kb SalI-SphI fragment from pNB2 | This study |
| pHS3952 | pBR328 carrying a 1.0-kb SspI-SphI fragment from pNB2 | This study |
| pHS941 | pBR328 carrying a 2.5-kb SspI-EcoRV fragment with nbzB from pNB2 | This study |
a
Abbreviations: NB+, growth on NB; NB−, no growth on NB; Apr, ampicillin resistance; Cmr, chloramphenicol resistance; Tcr, tetracycline resistance.