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. 2022 Jun 16;7(14):e158571. doi: 10.1172/jci.insight.158571

Figure 7. JAML regulates macrophage efferocytosis via a Mincle-dependent mechanism.

Figure 7

(A) Schematic diagrams showing the procedure of macrophage efferocytosis assay in vivo. FCM, flow cytometry. (B) Mice were injected intraperitoneally with PKH26-labeled apoptotic cells, and 45 minutes later lavage fluid was analyzed by flow cytometry for the percentage of F4/80+ macrophages that had incorporated the labeled neutrophils (n = 7). (C) Schematic diagrams showing the procedure of macrophage efferocytosis assay in vitro. (D) Overlay images of phase and fluorescence microscopy images of cultured BMDMs treated for 2 hours with UV-exposed PKH26-labeled Jurkat cells. Quantitative analysis of percentage of PKH26+ macrophages (n = 8). Data are mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. Two-way ANOVA test (B and D).