Freshly prepared dexamethasone stocks were compared to two-week dexamethasone in vitro eluate. Drug was assayed using real time polymerase chain reaction. Expression of interleukin-6 (IL-6) was used to assess anti-inflammatory activity. Actin expression was used as a load control. The columns are as follows: (−): no LPS, (+): + LPS, A: LPS + 0.1 μM fresh DEX, B: LPS + 1.0 μM fresh DEX, C: LPS + 0.1 μM eluted DEX, D: LPS + 1.0 μM eluted DEX. Data is presented as Mean ± Standard Deviation.