FIG. 2.

Mislocalization of NapA in TF140 and restoration of NapA export by tat genes (A) and specificity of the NapA detection system (B). The subcellular fractions are indicated: P, periplasm; M, membrane; C, cytoplasm. Nap activity (milliunits per milligram of protein) is given below the immunoblot. Note, the Nap activity in the periplasm is given as milliunits per gram (wet weight) of cells. (A) Lane 1, TF140; lane 2, TF140 harboring tatAB on pGE600; lane 3, TF140 harboring tatA on pGE601; lane 4, TF140 harboring tatB on pGE602. (B) Lane 1, partially purified NapA (arrow); lane 2, membrane of TF100 cells; lane 3, membrane of TF100 cells harboring the complete nap genes on pGE144 (35).