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Blocked nitrous oxide reduction in R. eutropha TF140 (A), which was restored by the expression of A. chroococcum tatA on pGE601 (B). Growth was performed anaerobically on fructose containing 10 mM nitrate, and the gaseous denitrification products were monitored by gas chromatography. ●, growth of R. eutropha culture at 436 nm; ■, nitrous oxide; ▴, dinitrogen. OD, optical density.
