FIG. 3.

Study of the effects of upstream sequences and IHF on transcription from the fusion promoters PRA1 (A) and PLA1 (B and C) by comparison of the levels of expression of C12O activity in the wild-type strain P. putida KT2442 and in its ihfA knockout derivative A8759. C12O activity was measured at different time points either in exponentially growing or stationary-phase cells of P. putida KT2442. Data (means ± standard deviations) of at least four independent experiments are presented. The growth curve of P. putida KT2442 in LB is shown (D). The growth rate of the strain A8759 is similar to that of KT2442 (not shown).