TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmid	Genotype or construction	Source or reference
E. coli
TG1	supE hsdΔ thiΔ (lac-proAB) F′	7
WM2015	subE thi Δ(lac-pro)	30
WM2017	WM2015 himA::TcrhimD::Cmr	30
S17-1 λ pir	Tpr SmrrecA thi pro (r− m+) RP4::2 Tc::Mu::Km Tn7 λ pir	33
P. putida
PaW85	Tn4652	2
KT2442	Tn4652 xylRS Pu-lacZ Rifr Smr	6
A8759	KT2442 Tn4652 ihfA::Kmr Pu-lacZ Rifr Smr	6
RT31	A8759 P. putida ihfA and ihfB under control of Ptac promoter and lacIq repressor Rifr Smr Tcr	This work
Plasmids
pBluescript SK	Cloning vector (Apr)	Stratagene
pEST1332	Plasmid pAYC32 carrying promoterless pheBA operon	29
pRA1	64-bp DraI-ClaI DNA fragment containing PRA1 promoter region cloned into pEST1332	36, Fig. 2
pRA1-7	82-bp HaeIII-ClaI DNA fragment containing PRA1 promoter region cloned into pEST1332	This work, Fig. 2
pRA1-12	288-bp Eco47III-ClaI DNA fragment containing PRA1 promoter region cloned into pEST1332	This work, Fig. 2
pLA1	110-bp DraI-ClaI DNA fragment containing PLA1 promoter region cloned into pEST1332	36, Fig. 2
pLA1-4	152-bp BstUI-ClaI fragment containing PLA1 promoter region cloned into pEST1332	This work, Fig. 2
pLA1-5	200-bp EcoRV-ClaI fragment containing PLA1 promoter region cloned into pEST1332	This work, Fig. 2
pLA1-12	387-bp HaeIII-ClaI fragment containing PLA1 promoter region cloned into pEST1332	This work, Fig. 2
pHip	Plasmid carrying E. coli ihfB	6
pMMB208	lacIq/Ptac-based broad-host-range expression plasmid (Kmr)	34
pUC18 Not	Identical to pUC18 but with NotI sites in multicloning region (Apr)	25
pUTmini-Tn5luxAB	Delivery plasmid for mini-Tn5 luxAB (Apr Tcr)	11
pUTtetPF	pUTmini-Tn5luxAB carrying P. putida ihfA and ihfB Ptac promoter and lacIq	This work
pUCPu130	129-bp DpnI fragment of Pu promoter region of xyl genes in TOL plasmid cloned into pUC18	26