TABLE 3.
exo and exp gene expression in different phosphate conditions in S. melilotia
Strain and fusion | Mean Miller units ± SD of β-galactosidase activity at:
|
||||
---|---|---|---|---|---|
0 mM phosphate | 20 mM phosphate | 100 mM phosphate | 150 mM phosphate | 200 mM phosphate | |
Rm8501 | |||||
exoY-lacZ | 8.1 ± 1.0 | 11 ± 0.7 | 37 ± 3.0 | 55 ± 4.0 | 120 ± 13 |
expA-lacZ | 10 ± 1.5 | 1.9 ± 0.1 | 1.4 ± 0.2 | 1.7 ± 0.1 | 1.3 ± 0.5 |
RmH615 | |||||
exoY-lacZ | 4.9 ± 1.0 | 8.5 ± 1.7 | 19 ± 2.0 | 98 ± 4.0 | 119 ± 13 |
expA-lacZ | 0.4 ± 0.1 | 0.5 ± 0.1 | 0.7 ± 0.2 | 0.9 ± 0.2 | 0.7 ± 0.2 |
Rm8501 is a lac mutant of Rm1021. RmH615 is a lac mutant and has a phoB mutation. All data are calculated in Miller units of β-galactosidase activity.