Figure 4 .

Loss of Tet2 mediated different immune response patterns in AR mice. (A) Global 5hmC content of nasal mucosa purified 100 and 50 ng DNA measured by immune-dot blot in Tet1^−/−, Tet1^+/−, Tet2^−/− and Tet2^+/− mice. Tet2^−/− mice gained 5hmC content globally in nasal mucosa. The density measurement was performed by ImageJ. (B) Identification of Tet2^−/− AR-induced dynamically hydroxymethylated gain-DhMRs and loss-DhMRs by DESeq2 (P-value < 0.05, absolute log 2 folds change >0.848). (C) Genomic annotation of DhMRs shows the percentage change of each genomic region. Tet2^−/− AR-induced mice show a decrease in the intergenic percentage and an increase in the intronic region percentage. (D) Panther pathway analyses of DhMRs in both the wildtype and Tet2^−/− groups.