FIG. 1.
Phenotypic effects of Gfp-MinD, MinD, and MinE1–53-Gfp. (Panels a to f) Strain PB114 (λDB217) (ΔminCDE [Plac-minC]) containing the indicated plasmids was grown at 30° for 3.5 h in L broth containing 1 mM IPTG and 0.01% arabinose unless otherwise indicated and then examined by phase-contrast microscopy. Panels: a, plasmid pBAD33 (vector); b, plasmid pSLR22 (Para-gfp::minD) (arabinose was omitted); c, plasmid pAS73 (Para-minD); d, plasmid pSLR22 (Para-gfp::minD); e, plasmid pSLR24 (Para-minD minE); f, plasmid pSLR23 (Para-gfp::minD minE); g, strain RC1/pYW3 (Plac-minC gfp::minD minE) was grown at 30° for 4.5 h in L broth containing 10 μM IPTG; h and i, strain DH5α/pFX1 (minC+D+E+/Plac-minE1–53::gfp) was grown for 4 h at 30° in L broth containing 0.05 mM IPTG (h) or 1 mM IPTG (i). Bars, 5 μm.