TABLE 1.
Effect of ORP on metabolic end product distribution for anaerobic batch cultivation in Trypticase soy broth medium maintained at a pH of 7.0
| Reducing compound added (concn [g/liter]) | NaBH4 (0.2) | None | DTT (1) |
|---|---|---|---|
| ORP during growth phase | −360 | −325 | −280 |
| Final biomass (g [dry weight]/liter) | 1.02 ± 0.05 | 1.15 ± 0.08 | 0.77 ± 0.03 |
| Formatea | 0.88 ± 0.08 | 1.16 ± 0.10 | 1.31 ± 0.08 |
| Ethanola | 0.87 ± 0.08 | 0.89 ± 0.12 | 0.94 ± 0.15 |
| Acetatea | 0.81 ± 0.05 | 0.88 ± 0.09 | 0.87 ± 0.05 |
| Succinatea | 0.09 ± 0.02 | 0.09 ± 0.02 | 0.11 ± 0.02 |
| Lactatea | 0.15 ± 0.02 | 0.07 ± 0.01 | 0.01 ± 0.01 |
| CO2b | 0.71 | 0.58 | 0.39 |
| Carbon recovery (%) | 96 | 98 | 97 |
| YATP (g [dry weight]/mole of ATP) | 7.0 | 7.9 | 5.6 |
a
Values (in moles per mole of consumed substrate) are averages (± standard deviations) from two different experiments.
b
Calculated using theoretical flux (CO2 = ethanol + acetate − formate − CO2 consumed by succinate production).