FIGURE 5.

SKI inhibited the RAS/Wnt/β-catenin signalling axis in AngII-induced HK-2 cells. (A) Protein expression of AT₁R in AngII-induced HK-2 cells treated with SKI and losartan. (B) Quantitative analysis of the protein expression of AT₁R in AngII-induced HK-2 cells treated with SKI and losartan. (C) Representative immunofluorescent analysis of Wnt1 and β-catenin in AngII-induced HK-2 cells treated with SKI. (D) Protein expression of Wnt1, active β-catenin and β-catenin, as well as its downstream target gene products, including Snail1, Twist, MMP-7, PAI-1, and FSP1, in AngII-induced HK-2 cells treated with SKI. (E) Quantitative analysis of Wnt/β-catenin signalling pathway protein expression in AngII-induced HK-2 cells treated with SKI. **p < 0.01 compared with the control group; ^# p < 0.05, ^## p < 0.01 compared with the AngII-induced group.