FIGURE 6.

Rhein inhibited EMT by blocking the RAS in AngII-induced HK-2 cells. (A) Cell viability analysis after HK-2 cells were treated with increasing concentrations of rhein (0–12.5 μM). (B) Protein expression of AT₁R in AngII-induced HK-2 cells treated with rhein and losartan. (C) Quantitative analysis of the protein expression of AT₁R in AngII-induced HK-2 cells treated with rhein and losartan. (D) Protein expression of collagen I, α-SMA, E-cadherin and fibronectin in AngII-induced HK-2 cells treated with rhein. (E) Quantitative analysis of the protein expression of collagen I, α-SMA, E-cadherin and fibronectin in AngII-induced NRK-49F cells treated with rhein. (F) Representative immunofluorescent analysis of fibronectin in AngII-induced HK-2 cells treated with SKI. (G) Protein expression of collagen I, α-SMA, E-cadherin and fibronectin in AngII-induced NRK-49F cells treated with rhein. (H) Quantitative analysis of the protein expression of collagen I, α-SMA, E-cadherin and fibronectin in AngII-induced NRK-49F cells treated with rhein. *p < 0.05, **p < 0.01 compared with the control group; ^# p < 0.05, ^## p < 0.01 compared with the AngII-induced group.