Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Affinity chromatography of FecR-(His)6 (A) and (His)10-FecR (B) on a Ni-NTA agarose column. The eluted fractions were subjected to SDS-PAGE (with 15% acrylamide gels) and stained with Coomassie brilliant blue. (C) Western blotting of Ni-NTA agarose column eluant fractions with anti-FecR serum. FecR-(His)6, (His)10-FecR, and their proteolytic cleavage products are indicated by arrows. Numbers indicate molecular masses in kilodaltons.
