TABLE 1.
E. coli strains and plasmids used in this study
| Strain or plasmid | Genotype | Source or reference |
|---|---|---|
| Strains | ||
| DH5α | Δ(argF lac)U196 endA1 recA1 hsdR17 (rK− mK+) supE44 thi-1 gyrA1 relA1 (F′ φ80 ΔlacZΔM15) | 8 |
| AA93 | Δfec aroB Δ(argF lac)U169 araD139 rspL150 relA1 deoC1 flbB5301 ptsF25 rbsR | 15 |
| BL21(DE3) | F−hsdS galT; phage T7 polymerase under lacUV5 control | 25 |
| SU202 | lexA71::Tn5 sulA211 sulA::lacZ Δ(lacIPOZYA)169 F′ lacIqlacZΔM15::Tn9 | 5 |
| Plasmids | ||
| pT7-7 | Phage T7 promoter; ori (ColE1) Apr | 26 |
| pHSG576 | pSC101 derivative; Cmr | 27 |
| pMLB1034 | ori (ColE1) Apr | 22 |
| pET25b(+) | Phage T7 promoter; C-terminal His-tag; ori (ColE1) Apr | Novagen |
| pET19b | Phage T7 promoter; N-terminal His-tag; ori (ColE1) Apr | Novagen |
| pFecRHis | pET25b(+) fecR | This study |
| pHisFecR | pET19b fecR | This study |
| pIS136 | pHSG576 fecI | 15 |
| pIS135 | pHSG576 fecI fecR | 15 |
| pIRHis | pHSG576 fecI fecRhis | This study |
| pIHisR | pHSG576 fecIhisR | This study |
| pIS1034 | pMLB1034 fecA; fecA-lacZ fusion | 15 |
| pSV66 | pHSG576 fecI fecR fecA | 9 |
| pAA70 | pT7-7 fecR | 29 |
| pAA71 | pT7-7 fecI | 1 |
| pIS711 | pT7-7 fecA | 10 |
| pSP47 | pT7-7 fecAΔ47–101 | 10 |
| pDP804 | LexA1–87408-Jun zipper | 5 |
| pMS604 | LexA1–87WT-Fos zipper | 5 |
| pUS10 | LexA1–87WT-FecA1–79a | This study |
| pUS11 | LexA1–87408-FecR101–317a | This study |
| pUS12 | LexA1–87408-FecR151–317a | This study |
| pUS13 | LexA1–87408-FecR101–275a | This study |
| pSM173 | LexA1–87WT-FecI1–173a | This study |
| pSM133 | LexA1–87WT-FecI1–133a | This study |
| pSM4 | LexA1–87WT-FecI82–173a | This study |
| pSM85 | LexA1–87408-FecR1–85a | This study |
| pSM9 | LexA1–87408-FecR9–85a | This study |
| pSM19 | LexA1–87408-FecR19–85a | This study |
| pSM38 | LexA1–87408-FecR1–38a | This study |
| pSM58 | LexA1–87408-FecR1–58a | This study |
a
Subscripts indicate the amino acid residues contained in the protein deletion derivatives.