TABLE 4.
β-Galactosidase activity by the bacterial two-hybrid Lex-based system in E. coli SU202 sulA::lacZa
| Protein combination | Percentage of β-galactosidase activity ± SD |
|---|---|
| LexA1–87408-Jun zipper | 100 ± 22 |
| LexA1–87WT-Fos zipper | 100 ± 18 |
| LexA1–87408-Jun zipper and LexA1–87WT-Fos zipper | 7 ± 3 |
| LexA1–87WT-FecA1–87 | 100 ± 6 |
| LexA1–87408-FecR101–317 | 100 ± 4 |
| LexA1–87WT-FecA1–79 and LexA1–87408-Jun zipper | 102 ± 6 |
| LexA1–87408-FecR101–317 and LexA1–87WT-Fos zipper | 88 ± 3 |
| LexA1–87WT-FecA1–79 and LexA1–87408-FecR101–317 | 9 ± 2 |
| LexA1–87WT-FecA1–79 and LexA1–87408-FecR151–317 | 124 ± 25 |
| LexA1–87WT-FecA1–79 and LexA1–87408-FecR101–275 | 107 ± 13 |
| LexA1–87WT-FecI1–173 | 85 ± 17 |
| LexA1–87408-FecR1–85 | 96 ± 14 |
| LexA1–87408-Jun zipper and LexA1–87WT-FecI1–173 | 113 ± 19 |
| LexA1–87WT-Fos zipper and LexA1–87408-FecR1–85 | 120 ± 12 |
| LexA1–87WT-FecI1–173 and LexA1–87408-FecR1–85 | 14 ± 4 |
| LexA1–87WT-FecI1–173 and LexA1–87408-FecR9–85 | 12 ± 4 |
| LexA1–87WT-FecI1–173 and LexA1–87408-FecR19–85 | 102 ± 19 |
| LexA1–87WT-FecI1–173 and LexA1–87408-FecR1–58 | 14 ± 5 |
| LexA1–87WT-FecI1–173 and LexA1–87408-FecR1–38 | 107 ± 10 |
| LexA1–87WT-FecI1–133 and LexA1–87408-FecR1–85 | 80 ± 21 |
| LexA1–87WT-FecI82–173 and LexA1–87408-FecR1–85 | 111 ± 23 |
a
The activity of the various protein combinations is shown as the relative percentage of β-galactosidase activity. An activity of 100% corresponds to approximately 250 Miller units. Percentage indicated is an average over three experiments; the standard deviation is shown.