TABLE 2.
Purification scheme of the DNA polymerase from P. islandicum
| Purification step | Total protein (mg) | Total activity (U) | Sp act (U/mg) | Purification (fold) | Recovery (%) |
|---|---|---|---|---|---|
| Crude extract | 45.0 | 1,138 | 25.3 | 1 | 100 |
| Heat denaturation | 11.5 | 768 | 66.8 | 2.6 | 68 |
| Streptactin chromatography | 0.4 | 502 | 1,255.0 | 49.6 | 44 |