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. 2000 Feb;182(3):655–663. doi: 10.1128/jb.182.3.655-663.2000

TABLE 2.

Purification scheme of the DNA polymerase from P. islandicum

Purification step Total protein (mg) Total activity (U) Sp act (U/mg) Purification (fold) Recovery (%)
Crude extract 45.0 1,138 25.3 1 100
Heat denaturation 11.5 768 66.8 2.6 68
Streptactin chromatography 0.4 502 1,255.0 49.6 44