TABLE 3.

Influence of inhibitors on the DNA polymerase activity from P. islandicum

Inhibitor	Concentrationa	DNA polymerase activity (%)b
NEMc	0	100
	1	40
	2	30
	5	0
Aphidicolin	0 (0)	100
	50 (17)	100
	200 (68)	70
	500 (170)	55
	2,000 (680)	21
ddGTP	5	95
	10	80
	20	53

^aThe concentrations of the NEM inhibitor are millimolar, and the concentrations of the aphidicolin inhibitor are micromolar and, in parentheses, micrograms per milliliter. The concentrations of the ddGTP inhibitor are ratios of ddGTP/dGTP. 

^bThe influence of different inhibitors was detected at standard assay conditions (pH 7.3; 0.5 mM MgCl₂; 0 mM KCl; 75°C) using 0.2 U of purified DNA polymerase. 

^cA preincubation of the enzyme with NEM was performed at 90°C for 20 min.