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. Author manuscript; available in PMC: 2022 Aug 31.
Published in final edited form as: Nat Aging. 2022 Aug 11;2(8):714–725. doi: 10.1038/s43587-022-00261-5

Extended Data Fig. 9 |. Sequencing depth and down-sampling performance for cardiomyocytes and neurons.

Extended Data Fig. 9 |

A, No systematic difference in sequencing depth (p = 0.51, two-tailed Wilcoxon test) between tetraploid cardiomyocytes (n = 48) and neurons (n = 155). Eight outlier cardiomyocytes (in the dashed rectangle) were intentionally sequenced at doubled sequencing depth from two donors. Boxplot with whisker denotes minimum, 25%, median, 75% quartiles, and maximum. B, LiRA-estimated sSNV burden remained generally robust with varied sequencing depths at or above the average depth in neurons, denoted by the dashed line. Four outlier cardiomyocytes from (A) were randomly chosen, and their sequencing reads were in silico down-sampled into 25%, 50%, and 75% of the original sequencing depths. Error bar, mean ± 95%CI.