Table 1. Statistics analysis of each constructed library via NGS.

Libraries	Total read counts	Mapped read counts	Read counts > 0		Read counts ≥ 20
			sgRNA	Gene*	sgRNA	Gene†
			(ratio‡)	(ratio)	(ratio)	(ratio)
Plasmid (NGG)	10646744	9582402	7466	2837	7220	2812
			97.61%	97.76%	94.39%	96.90%
Plasmid (NGA)	7673608	6963096	3887	2024	3868	2020
			99.46%	98.54%	98.98%	98.34%
Transformant 1	10660732	10112918	11265	3036	10821	3033
			97.47%	99.84%	93.63%	99.74%
Transformant 2	11342596	10776907	11330	3035	10868	3033
			98.03%	99.80%	94.04%	99.74%
Transformant 3	9731896	9249060	11256	3039	10882	3035
			97.40%	99.93%	94.16%	99.80%
Inactivation 1	9111468	8606431	10931	3033	10528	3029
			94.58%	99.74%	91.10%	99.61%
Inactivation 2	9746060	9224673	11046	3034	10529	3029
			95.58%	99.77%	91.10%	99.61%
Inactivation 3	8551616	8088598	10929%	3033	10566	3027
			94.57%	99.74%	91.43%	99.54%

*Number of genes with at least one sgRNA that read count >0.

†Number of genes with at least one sgRNA that read count ≥20.

‡Mapping ratio to the in silico library.