Fig. 5. scRNA-seq analysis of Jag1-regulated endothelial heterogeneity.

Aortas from Jag1^ECKO and control mice were analyzed by FACS of CD31⁺ CD45⁻ cells coupled to scRNA-seq. (A) t-SNE representation of single-cell transcriptomes from Jag1^ECKO and control mice colored by cluster assignment. Clusters were identified using unbiased hierarchical clustering. (B) t-SNE showing the cell contribution of Jag1^ECKO and control mice to each subpopulation. (C) Bar graph showing the cell distribution of Jag1^ECKO and control mice across cell clusters, by percentage (left) and number of cells (right). Clusters 4 to 6 are largely composed of ECs derived from Jag1^ECKO mice, whereas cluster 2 and 7 to 11 are mainly composed of ECs derived from control mice. (D) Representation of enriched GO pathways on the t-SNE map. An overall score summarizing the expression of genes in each GO pathway is calculated for every cell in the scRNA-seq dataset. Differences between means were calculated using a Wilcoxon signed-rank test.