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. 2022 Aug 31;8(35):eabn0356. doi: 10.1126/sciadv.abn0356

Fig. 6. Nitration of mitochondrial NDUFB8 during neuronal hyperactivity in the absence of h-αS overexpression.

Fig. 6.

(A) iR26-αS mice were injected with AAVs delivering (nonfloxed) hM3D fused with mCherry under control of the human synapsin promoter. (B and C) Mice received an intravagal injection of hM3D-AAVs and were then treated with daily injections of either saline (n = 5, gray bar) or CNO (n = 5, black bar) during weeks 4 and 5 after AAV injection. They were then sacrificed at the end of week 5. Medullary tissue sections were processed for fluorescent microscopy and double-labeled with 3-NT/NDUFB8 PLA and anti-RFP (for the detection of mCherry-tagged hM3D). Representative confocal images show fluorescent PLA dots within hM3D/RFP-positive DMnX neurons. Scale bar, 10 μm (B). Fluorescent intensity was measured within hM3D/RFP-positive DMnX neurons. Approximately 40 neurons per animal were analyzed, and for each animal, intensity values were averaged and calculated as percentage of the mean value in the saline group. Box and whisker plots show median, upper and lower quartiles, and maximum and minimum as whiskers. *P < 0.05 (C).