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Purification of gp3. SDS-polyacrylamide gels of gp3 at various stages of purification are shown. Lane A, supernatant of the cell lysate after sedimentation; lane B, ammonium sulfate precipitate; lane C, purified gp3 after DEAE–Sephadex A-50 and Superdex-75 column chromatographies; lane D, molecular mass markers. The arrow denotes the 55-kDa band of gp3.
