TABLE 1.
Bacterial strains, phages, and plasmids
| Strain, phage, or plasmid | Genotype or characteristic(s) | Reference or source |
|---|---|---|
| E. coli K-12 strains | ||
| CR63 | supD60 (Su+) | Lab strain (F.A.E.) |
| CAJ70 | supUGA (Su+) | 40 |
| K-12(λ)/s | sup0 (Su−) | 11 |
| E. coli B strains | ||
| Bb | sup0 (Su−) | Lab strain (E.B.G.) |
| Be | sup0 (Su−) | Lab strain (F.A.E.) |
| B40Su− | argF40(Am) sup0 (Su−) | Lab strain (F.A.E.) |
| B40SuI+ | argF40(Am) supD60 (Su+) | Lab strain (F.A.E.) |
| B40SuII+ | argF40(Am) supE44 (Su+) | Lab strain (F.A.E.) |
| B40SuIII+ | argF40(Am) supF66 (Su+) | Lab strain (F.A.E.) |
| HB101 | F−hds20(rB− mB−) recA13 ara-14 ΔproA3 lacY1 galK2 rpsL20(Smr) xyl-5 mtl-1 supE44 leuB6 | 4 |
| BL21(λDE3) | F−hsdS(rB− mB−) gal (int::PlacUV5-T7 gene 1 imm 21 min5) | 44;1; |
| Phage strains | ||
| T4D+ | Wild type | Lab strain |
| T4 3− NG418 | Mutation in gene 3 | Lab strain (F.A.E.) |
| T4 3− NG131 | Mutation in gene 3 | Lab strain (F.A.E.) |
| T4 19opC95 | Opal mutation in gene 19 | W. B. Wood |
| T4 10amB255 | Amber mutation in gene 10 | Lab strain (F.A.E.) |
| T4 3+/15−/23−/34− 15amN133/23amH11/34amB25 | Defective in head formation (gene 23amH), tail fiber formation (gene 34amB25), and tail completion (gene 15amN133) | Lab construct (11) |
| T4 3−/15−/23−/34− NG418/15amN133/23amH11/34amB25 | Defective in head formation (gene 23amH11), tail fiber formation (gene 34amB25), and tail completion (gene 15amN133 and gene 3amNG418) | This work |
| M13mp7 | M13 derivative with polylinker containing an inverted repeat | 33 |
| Plasmids | ||
| pTFP2110 | pBR322 derivative carrying in the PstI site a 2.8-kb insert containing T4 phage genes 3 to 53 | 49 |
| pET9 | pBR322 derivative used for gene expression; Kanr | 45 |
| pLysS | pACYC184 derivative carrying T7 gene 3.5 (coding for T7 lysozyme); Cmr | 43 |
| pAVgp3 (pAV9 · 3 · 1) | pET9 derivative expressing T4 gene 3; Kanr | This work |