Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) | Mertk | NCBI Gene ID | 17289 | |
| Gene (M. musculus) | Tyro3 | NCBI Gene ID | 22174 | |
| Strain, strain background (M. musculus, males and females) | C57BL/6J | The Jackson Laboratory | Strain# 000664 | |
| Genetic reagent (M. musculus) | Mertk -/-V1 | Lu et al., 1999 | Both males and females used | |
| Genetic reagent (M. musculus) | Mertk -/-V2 | This paper | See section ‘Animals’, both males and females used | |
| Genetic reagent (M. musculus) | Mertk -/-V3 | This paper | See section ‘Animals’, both males and females used | |
| Genetic reagent (M. musculus) | Tyro3 -/- | Lu et al., 1999 | Both males and females used | |
| Genetic reagent (M. musculus) | Mertk -/-V2 Tyro3 -/-V2 | This paper | See section ‘Animals’, both males and females used | |
| Biological sample (M. musculus) | Retinal pigment epithelial cells | This paper | Freshly isolated from indicated mouse strains, see ‘Materials and methods’ sections for various applications | |
| Biological sample (M. musculus) | Bone marrow-derived macrophages | This paper | Freshly isolated from indicated mouse strains, see ‘Materials and methods’ sections for various applications | |
| Antibody | Anti-MERTK (rabbit polyclonal) |
Abcam | Cat# ab95925 | WB (1:1000) |
| Antibody | Anti-TYRO3 (rabbit monoclonal) |
Cell Signaling Technology | Cat# 5585S | WB (1:1000) |
| Antibody | Anti-β-actin (rabbit monoclonal) |
Cell Signaling Technology | Cat# 8457S | WB (1:10,000) |
| Antibody | Anti-β-actin (mouse monoclonal) |
Cell Signaling Technology | Cat# 3700S | WB (1:10,000) |
| Antibody | Anti-rabbit IgG, IRDye800 (donkey polyclonal) |
Li-COR Biosciences | Cat# 926-32213 | WB (1:15,000) |
| Antibody | Anti-mouse IgG, IRDye680 (donkey polyclonal) |
Li-COR Biosciences | Cat# 926-68072 | WB (1:20,000) |
| Antibody | Anti-mouse CD16/32 (rat monoclonal) | BioLegend | Cat# 101302 | FC (1:1000) |
| Antibody | Anti-mouse CD11b (rat monoclonal) | BioLegend | Cat# 101222 | FC (1:200) |
| Antibody | Anti-mouse F4/80 (rat monoclonal) | BioLegend | Cat# 123114 | FC (1:200) |
| Antibody | Anti-mouse MERTK (rat monoclonal) | Invitrogen | Cat# 17-5751-82 | FC (1:200) |
| Chemical compound, drug | RNAprotect Cell Reagent | QIAGEN | Cat# 76526 | |
| Chemical compound, drug | Paraformaldehyde 16% | Electron Microscopy Sciences | Cat# 15710 | |
| Chemical compound, drug | Glutaraldehyde 25% | Electron Microscopy Sciences | Cat# 16200 | |
| Chemical compound, drug | Tropicamide ophthalmic Solution 0.5% | Sandoz | Cat# 61214-354-01 | |
| Chemical compound, drug | cOmplete Protease Inhibitor Cocktail | Roche | Cat# 11697498001 | |
| Chemical compound, drug | Eye Fixative | ServiceBio | Cat# G1109 | |
| Commercial assay, kit | RNeasy Plus Micro Kit | QIAGEN | Cat# 74034 | |
| Commercial assay, kit | iScript cDNA Synthesis Kit | Bio-Rad | Cat# 1708891 | |
| Commercial assay, kit | Mini-PROTEAN TGX Stain-Free Precast Gel | Bio-Rad | Cat# 4568025, 4568125 | |
| Commercial assay, kit | Immun-Blot PVDF membrane | Bio-Rad | Cat#1620177 | |
| Commercial assay, kit | Pierce BCA Protein Assay Kit | Thermo Scientific | Cat# 23227 | |
| Other | Hematoxylin-eosin staining | iHisto histopatholgy support | https://www.ihisto.io/ |