Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
|
Gene (Homo sapiens) |
AAVS1 | GenBank | HGNC:HGNC:22 | |
|
Gene (Homo sapiens) |
VEGFA | GenBank | HGNC:HGNC:12680 | |
|
Gene (Homo sapiens) |
EMX1 | GenBank | HGNC:HGNC:3340 | |
|
Gene (Homo sapiens) |
GRIN2B | GenBank | HGNC:HGNC:4586 | |
| Recombinant DNA reagent | Instant Sticky-end Ligase Master Mix | NEB | Catalog #: M0370S | |
| Recombinant DNA reagent | T4 DNA ligase | NEB | Catalog #: M0202S | |
| Cell line (Homo-sapiens) | HEK293T (normal, Adult) |
ATCC | CRL-3216 | |
| Cell line (Homo-sapiens) | HeLa | ATCC | CRM-CCL-2 | |
| Cell line (Homo-sapiens) | SH-SY5Y | ATCC | CRL-2266 | |
| Cell line (Homo-sapiens) | A375 (normal, Adult) |
ATCC | CRL-1619 | |
| Cell line (Homo-sapiens) | HCT116 (adult male) |
ATCC | CCL-247 | |
| Cell line (Mus musculus) | N2a cells (mouse neuroblasts) |
ATCC | CCL-131 | |
| Antibody | Anti-HA (rabbit polyclonal) | abcom | abcam: ab137838; RRID:AB_262051 | (1:1000) |
| Antibody | Anti-GAPDH (rabbit polyclonal) | Cell Signaling | Cell Signaling:#3683; RRID:AB_307275 | (1:1000) |
| Sequence-based reagent | dsODN-F | This paper | dsODN oligo primer | 5ʹ- P-G*T*TTAATTGAGTTGTCATATGTTAATAACGGT*A*T - 3ʹ |
| Sequence-based reagent | dsODN-R | This paper | dsODN oligo primer | 5ʹ- P-A*T*ACCGTTATTAACATATGACAACTCAATTAA*A*C –3ʹ |
| Sequence-based reagent | P5_index_F | This paper | PCR primers | AATGATACGGCGACCACCGAGATCTACACTGAACCTTA CACTCTTTCCCTACACGAC |
| Sequence-based reagent | Nuclease_off_+_G SP |
This paper | PCR primers | GGATCTCGACGCTCTCCCTATACCGTTATTAACATATGACA |
| Sequence-based reagent | Nuclease_off_- _GSP1 |
This paper | PCR primers | GGATCTCGACGCTCTCCCTGTTTAATTGAGTTGTCATATGTTAATAAC |
| Sequence-based reagent | P5_2 | This paper | PCR primers | AATGATACGGCGACCACCGAGATCTACAC |
| Sequence-based reagent | Nuclease_off _+_GSP2 |
This paper | PCR primers | CAAGCAGAAGACGGCATACGAGATTCGCCTTAGTGACTGGAGT TCAGACGTGTGCTCTTCCGATCTACATATGACAACTCAATTAAAC |
| Sequence-based reagent | Nuclease_off _- _GSP2 |
This paper | PCR primers | CAAGCAGAAGACGGCATACGAGATCTAGTACGGTGAC TGGAGTCCTCTCTATGGGCAGTCGGTGATTTGAGTTG TCATATGTTAATAACGGTA |
| Software, algorithm | FlowJo software | FlowJo VX | ||
| Software, algorithm | CorelDRAW 2020 software | CorelDRAW 2020 | ||
| Software, algorithm | Vector NTI software | Vector NTI | ||
| Software, algorithm | GraphPad Prism software | GraphPad Prism 7 (https://graphpad.com) | RRID:SCR_015807 | Version 7.0.0 |
| Chemical compound, drug | SYBR Gold nucleic acid stain | Thermo Fisher Scientific | Thermo Fisher Scientific: S11494 |