Skip to main content
. 2022 Aug 31;11:e78012. doi: 10.7554/eLife.78012

Figure 1. Cancer tropism of 3-chloropiperidines (3-CePs) is not explained by DNA damage.

(A) Chemical structure of the analyzed 3-CePs (M=monofunctional and B=bifunctional). (B) Quantification of genomic DNA damage in BxPC-3 and HCT-15 cells treated with M (10 nM and 100 nM), B (200 nM and 2 µM), or DMSO (dimethyl sulfoxide) 0.5% (ctrl) for 6 hr and analyzed by the fast micromethod single-strand-break assay: alkaline denaturation of DNA is followed in time up to 20 min by monitoring the fluorescence of the dsDNA-specific PicoGreen dye. (C) Cell cycle distribution (accumulation in G1 vs G2/S phases) of BxPC-3 and HCT-15 cells treated with M (10 nM), B (200 nM), or DMSO 0.5% for 6 hr and 72 hr analyzed by FACS (Fluorescence Activated Cell Sorting). At least three biological replicates were obtained per condition and unpaired two-tailed Student’s t-test was performed to assess statistical significance (p<0.05). (D) Analysis of H2AX phosphorylation in BxPC-3 and HCT-15 cells treated with M (10 nM), B (200 nM), or DMSO 0.5% for 6 hr and 12 hr analyzed by FACS. At least three biological replicates were obtained per condition, and unpaired two-tailed Student’s t-test was performed to assess statistical significance (p<0.05). (E) Schematic representation of the adopted omic-based approach.

Figure 1.

Figure 1—figure supplement 1. Cancer tropism of 3-CePs is not explained by DNA damage—supporting data.

Figure 1—figure supplement 1.

(A) Flow cytometry gating strategy for the cell cycle analysis and γH2AX induction. (B) Cell cycle distribution (accumulation in G1 vs G2/S phases) of BxPC-3 and HCT-15 cells treated with M (10 nM), B (200 nM), or DMSO 0.5% for 12 hr analyzed by FACS. At least three biological replicates were obtained per condition, and unpaired two-tailed Student’s t-test was performed to assess statistical significance (p<0.05).