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. 2022 Aug 18;11:e77032. doi: 10.7554/eLife.77032

Figure 3. The role of the transmembrane (TM) domain as a chaperone for helical domain (HD)/adenylyl cyclase (AC) assembly.

Figure 3.

Guide rail-like structures are formed by the TM1–5 in Cya (black, A) and the TM1–5/TM7–11 in AC9 (white, B). The arrangement of these rail-like structures positions the TM6 helices for optimal assembly of the HD and the catalytic domain. (C) The views of Cya and AC9 with the TM1–5 (Cya) and TM1–5/TM7–11 (AC9; PDB ID: 6r3q) represented as transparent surface. (D) The TM–HD regions of Cya and AC9 aligned. Despite the difference in HD length and the deviation in the TM domains, the cores of the HD domains are well aligned. (E, F) Similar to C, D, for the human soluble guanylyl cyclase sGC (inactive form; PDB ID: 6jt0). (G, H) Similar to E, F, for the activated form of sGC (sGC*; PDB ID: 6jt2). The brackets in F and H indicate the misaligned (F) and aligned (H) portions of the Cya and sGC HDs.