Figure 7. Abundance of private and public TCRα and TCRβ sequences in CD4⁺ and CD8⁺ T cells and changes with age.

(A) Abundance of private (found in 1 donor only) and public (found in 2–30 donors) TCRα (top) and TCRβ (bottom) sequences of CD4⁺ T cells. Black dots are number of UMIs for 1 TCRα or TCRβ sequence. Red dots are median values for TCRs that share the same number of donors (in log₁₀). (B) Abundance of private and public TCRα (top) and TCRβ (bottom) sequences of CD8⁺ T cells. (C) Alteration of the abundance of private and public TCRα and TCRβ sequences of CD4⁺ T cells with age. Private and public TCRα and TCRβ sequences of CD4⁺ T cells for each donor. Each sample is presented as percentage of total TCRα and TCRβ sequences (UMI counts). Thin short lines link 2 donations from 1 individual. Thick trend lines were calculated by MLE analysis. The colored shade around the trend line indicates the 95% confidence interval. Slope (S) and P values are provided. (D) Alteration of the abundance of private and public TCRα and TCRβ sequences of CD8⁺ T cells with age.