KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
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| Antibodies | ||
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| Rabbit-anti-mCherry | Abcam | Cat# ab167453 RRID: AB_2571870 |
| Rabbit-anti-c-Fos | Abcam | Cat# ab190289 RRID: AB_2737414 |
| Goat polyclonal anti c-Fos | Abcam | Cat# ab156802 RRID:AB_2747692 |
| Goat Anti-GFP antibody (FITC) | Abcam | Cat# ab6662 RRID: AB_305635 |
| FITC Anti-Biotin antibody | Abcam | Cat# ab53469 RRID:AB_867862 |
| Rabbit-anti-GLP-1 R | Abcam | Cat# ab218532 RRID: AB_2864762 |
| Rabbit-anti-GLP1 | Abcam | Cat# ab22625 RRID: AB_447206 |
| Goat-anti-ChAT | Millipore | Cat# AB144 RRID: AB_90650 |
| Rabbit Anti-DBH | Abcam | Cat# ab209487 RRID: AB_2892178 |
| Goat Anti-Nos1 | Abcam | Cat# ab1376 RRID: AB_300614 |
| Rabbit Anti-NeuN (Alexa647) | Abcam | Cat# ab190565 RRID:AB_2732785 |
| Alexa Fluor® 594 AffiniPure Donkey Anti-Rabbit IgG | Jackson Immuno Research Labs | Cat# 711–585-152 RRID:AB_2340621 |
| TRITC-conjugated affinipure Goat anti-Rabbit IgG (H+L) | Jackson Immuno Research Labs | Cat# 111–025-144 RRID: AB_2337932 |
| FITC-conjugated affinipure goat anti-rabbit IgG (H+L) | Jackson Immuno Research Labs | Cat# 111–095-144 RRID: AB_2337978 |
| FITC-conjugated affinipure Donkey AntiGoat IgG (H+L) | Jackson Immuno Research Labs | Cat# 705–095-147 RRID: AB_2340401 |
| TRITC-conjugated affinipure Donkey AntiGoat IgG (H+L) | Jackson Immuno Research Labs | Cat# 705–025-147 RRID: AB_2340389 |
| Biotinylated Goat Anti-Rabbit IgG antibody | Vector Laboratories | Cat# BA-1000 RRID: AB_2313606 |
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| Chemicals, peptides, and recombinant proteins | ||
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| CCK-SAP | Advanced Targeting Systems | Cat # IT-31 |
| Blank-SAP | Advanced Targeting Systems | Cat# IT-21 |
| Anti-DBH-SAP | Advanced Targeting Systems | Cat # IT-03 |
| Mouse IgG-SAP | Advanced Targeting Systems | Cat# IT-18 |
| FluoroGold (FG) | Fisher scientific | Cat# NC0560981 |
| CCK8 | AnaSpec | Cat # AS-20739 |
| GLP-1 (7–36) | AnaSpec | Cat # AS-22462 |
| Biotin-labeled GLP-1 (7–36) | AnaSpec | Cat # AS-23586 |
| Exendin-9, Exendin (9–39) | AnaSpec | Cat # AS-24467 |
| Exendin-4 | AnaSpec | Cat # AS-24463 |
| FAM-labeled Exendin-4 | AnaSpec | Cat # AS-60280–05 |
| Intralipid 20%, emulsion | SigmaAldrich | Cat# I141 |
| Cellulose | SigmaAldrich | Cat # 435236–250G |
| Diphtheria Toxin | SigmaAldrich | Cat # D0564–1 MG |
| IntraLipid 20%, emulsion | SigmaAldrich | Cat# I141–100ML |
| Sucralose | SigmaAldrich | Cat # 69293–100G |
| Clozapine N-oxide (CNO) | Enzo Life Sciences | Cat # BML-NS105–0025 |
| Cholic Acid | Alfa Aesar | Cat # A11257 |
| Hoechst 33342 | Invitrogen | Cat # H3570 |
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| Critical commercial assays | ||
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| VECTASTAIN Elite ABC HRP Kit (Peroxidase, Standard) | Vector Laboratories | Cat# PK-6100 |
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| Experimental models: Organisms/strains | ||
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| Mouse: C57BL/6J (B6) | The Jackson Laboratory | JAX: 000664 |
| Mouse: Glp1rtm1.1(cre)Lbrl/RcngJ (Glp1r-ires-Cre) | The Jackson Laboratory | JAX: 029283 |
| Mouse: B6; 129S-Gcgtm1.1(icre)Gkg/J (GcgiCre) | The Jackson Laboratory | JAX:030663 |
| Mouse: B6.Cg-Gt(ROSA) 26Sortm32CAG-COP4*H134R/EYFP)Hze/J (Ai32) | The Jackson Laboratory | JAX: 024109 |
| Mouse: B6N;129-Tg(CAG-CHRM3*,-mCi-trine)1Ute/J (CAG-LSL-Gq-DREADD) | The Jackson Laboratory | JAX: 026220 |
| Mouse: Slc17a6tm2(cre)Lowl/J (Vglut2-ires-cre) | The Jackson Laboratory | JAX: 016963 |
| Mouse: B6;129S-Slc17a7tm1.1(cre;Hze/J (Vglut1-IRES2-Cre-D) | The Jackson Laboratory | JAX:023527 |
| Mouse: C57BL/6-Gt(ROSA) 26Sortm1(HBEGF)Awai/J (B6-iDTR) | The Jackson Laboratory | JAX:007900 |
| Mouse: B6.129S-Chattm1(cre)Lowl/MwarJ (B6J.ChAT-IRES-Cre::Δneo) | The Jackson Laboratory | JAX: 031661 |
| Mouse: B6.129-Nos1tm1(cre)Mgmi/J (Nos1cre) | The Jackson Laboratory | JAX: 017526 |
| Mouse: B6.Cg-Gt(ROSA) 26Sortm9(CAG-tdTomato)Hze/J (Ai9) | The Jackson Laboratory | JAX:007909 |
| Mouse: B6.Cg-Igs7tm148.1(tetO-GCaMP6f,CAG-tTA2)Hze/J (Ai148D) | The Jackson Laboratory | JAX: 030328 |
| Mouse: B6.;129-Gt(ROSA) 26Sortm5(CAG-Sun1/sfGFP)Nat/J (CAG-Sun1/sfGFP) | The Jackson Laboratory | JAX: 021039 |
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| Recombinant DNA | ||
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| AAV2-retro.hSyn.Cre | Dr. Wilson-Addgene AAV Viral Service | Addgene Viral Prep: 105553-AAVrg |
| AAV2-retro-hSyn.HI.EGFP-Cre | Dr. Wilson-Addgene AAV Viral Service | Addgene Viral Prep: 105540-AAVrg |
| AAV2-retro-hSyn-EGFP | Dr. Roth-Addgene AAV Viral Service | Addgene Viral Prep: 50465-AAVrg |
| AAV1 hSyn FLEx mGFP-2A-Synaptophysin-mRuby | Dr. Luo-Addgene AAV Viral Service | Addgene Viral Prep: 71760-AAV1 |
| AAV1-synP-FLEX-splitTVA-EGFP-B19G | Dr. Wickersham-Addgene AAV Viral Service | Addgene Viral Prep: 52473-AAV1 |
| AAV5-EF1a-DIO-hChR2(H134R)-EYFP | Dr. Deisseroth-Addgene AAV Viral Service | Addgene Viral Prep:20298-AAV5 |
| AAV1/AAV5-Ef1a-DIO-EYFP | Dr. Deisseroth-Addgene AAV Viral Service | Addgene Viral Prep:27056-AAV5 |
| AAV1-syn-FLEX-jGCaMP8m | GENIE Project Lab-Addgene AAV Viral Service | Addgene Viral Prep:162378-AAV1 |
| AAV-DJ-hSyn-DIO-rM3D(Gs)-mCherry | Dr. Roth - Duke Viral Vector Core | Addgene Plasmids: 50458 |
| AAV-DJ-hSyn-DIO-hM4D(Gi)-mCherry | Dr. Roth - Duke Viral Vector Core | Addgene Plasmids: 44362 |
| AAV-DJ-hSyn-DIO-mCherry | Dr. Roth - Duke Viral Vector Core | Addgene Plasmids: 50459 |
| AAV-DJ-hSyn-DIO-EGFP | Dr. Roth - Duke Viral Vector Core | Addgene Plasmids: 50457 |
| AAV1 /AAV5-flex-taCasp3-TEVp | Dr. Shah - University of North Carolina’s Vector Core | Addgene Plasmids: 45580 |
| H129 CAG-LSL-TK-tdTomato | NIH Center for Neuroanatomy with Neurotropic Virus | ΔTK-TT |
| H129 CMV-EGFP | NIH Center for Neuroanatomy with Neurotropic Virus | 414 |
| PRV CAG-DIO-TK-GFP | NIH Center for Neuroanatomy with Neurotropic Virus | Ba-2017 |
| PRV CMV-EGFP | NIH Center for Neuroanatomy with Neurotropic Virus | 152 |
| EnvA G-Deleted Rabies-mCherry | Viral Vector Core - Salk Institute for Biological Studies | 32636 |
| EnvA G-Deleted Rabies-eGFP | Viral Vector Core - Salk Institute for Biological Studies | 32635 |
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| Deposited data | ||
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| Behavioral, electrophysiological, anatomy, and analyzed intravital imaging rare data | Mendeley Data.com | https://doi.org/10.17632/zkjm36wp8s.1 |
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| Software and algorithms | ||
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| Matlab R20 17b | MathWorks | http://www.mathworks.com/products/matlab/ |
| PatchMaster 2.20 | HEKA | http://www.heka.com/index.html |
| Igor Pro 6.36 | WaveMetrics | https://www.wavemetrics.com/ |
| EthoVision XT 11.5 | Noldus | http://www.noldus.com/animal-behavior-research/products/ethovision-xt |
| LabView 2014 | LabView | http://www.ni.com/download/labview-development-system-2014/4735/en/ |
| GraphPad Prism 9 | GraphPad | http://www.graphpad.com/scientific-software/prism/ |
| Adobe design standard CS6 | Adobe | http://shop.adobe.com/ |
| IBM SPSS statistics 24.0 | IBM Predictive Software | https://www.ibm.com/support/knowledgecenter/SSLVMB |
| Win Movie Maker | VideoWinSoft Software | http://www.VideoWinSoft.com |
| Axograph X | AxoGraph | http://www.axograph.com |
| ImageJ | NIH | https://imagej.nih.gov/ij/ |
| Python 3 | Python Software Foundation. | https://www.python.org/ |
| OpenCV 4.5.3 | OpenCV team | https://opencv.org/ |
| Ffmpeg n4.4 | FFmpeg team | https://www.ffmpeg.org/ |
| NoRMCorre: Non-Rigid Motion Correction | Pnevmatikakis and Giovannucci (2017) | https://github.com/flatironinstitute/NoRMCorre |
| CalmAn-MATLAB | Pnevmatikakis et al. (2016); Giovannucci etal. (2019) | https://github.com/flatironinstitute/CaImAn-MATLAB |
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| Other | ||
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| Totally Light 2Go - non-caloric powdered flavor drink mix, cranberry and wild berry flavors; sugar-free, Splenda-sweetened | 4C Foods Corp. | N/A |
| Implantable Optical Fibers | Doric Lenses, Canada | MFC_200/240–0.22_6mm_ZF1.25(G)_FLT |
| ICV Single Guide Cannula, 24 Gauge Stainless-Steel, Short Pedestal, Cut Length 6mm | P1 Technologies | Cat # C316GS |
| ICV Single Dummy Cannula, 24 Gauge Stainless-Steel, Fit On Cap | P1 Technologies | Cat # C316FD |
| ICV Single Internal Cannula, 31 Gauge Stainless-Steel, Short Pedestal | P1 Technologies | Cat # C316IS |
| Homeothermic Monitoring System | Harvard Apparatus | Cat # 55–7020 |
| Nanofil Application Kits (Microinjection) | World Precision Instruments | Cat # Beveled (IO-KIT) |
| NanoFil Needles (Microinjection) | World Precision Instruments | Cat # Beveled, 36G (NF36BV-2) |
| Pump 11 Elite Nanomite (Microinjection) | Harvard Apparatus | Cat # 70–4507 |
| Spinal Cord Hook (Vagal operation) | Fine Science Tools | Cat #10162–12 |
| Double Ended Periosteal Elevator (Spinal operation) | GerVetUSA | Cat # GD50–1720 |
| Mouse Spinal Adaptor (Spinal operation) | Stoelting | Cat #51690 |
| Mouse Transverse Clamps (Spinal operation) | Stoelting | Cat #51691 |
| Delicate Suture Tying Forceps (Microsurgery) | Fine Science Tools | Cat# 11063–07 |
| Formvar-Insulated Nichrome Wires (EMG recording) | A-M system | Cat# 761000 |
| Microwire connections (EMG recording) | Neuralynx | Cat # EIB-16 |
| 16 tungsten microwires, 35-μm diameter (Electrophysiological recordings) | TDT systems | Cat # OMN1005 |
| Masterflex® Nylon, Female Luer (Fistula) | Cole-Parmer | Cat # UX-45502–20 |
| Masterflex®, Polypropylene, Male Luer Lock (Fistula) | Cole-Parmer | Cat # EW-30800–30 |
| Round Cover Slip German Glass #1.5, 15 mm (Intravital microscopy) | Electron Microscopy Sciences | Cat #64–0713 |
| Ultra Gel Control Super Glue (Intravital window) | Loctite | Cat # 45208 |
| Stainless steel large penny fender washers M12×24×1.5mm (Intravital microscopy) | Dywishkey | Cat # 304 |
| Low Toxicity Silicone Adhesive (Intravital microscopy) | WPI | Cat # KWIK-SIL |
| FluoroDish Cell Culture Dish 50mm | WPI | Cat # FD5040–100 |
| Catheter for mouse jugular vein, 1 to 3Fr, 10.5cm, collar @1.2cm. Fits 22ga. (Gastric balloon) | Instech laboratories | Cat # C10PU-MJV1403 |
| MicroRenathane tubing (OD 0.025”) (Ileo catheterization) | Braintree Scientific | Cat # MRE025 |
| Balloon Latex Balloon size 4 (0.06ML) (Gastric balloon) | Harvard Apparatus | Cat # PY2 73–3479 |
| Silicon probes | Neuronexus | Cat # A2×2-tet-10mm-150–150-121-A16 |
| Headplates | Mt. Sinai Machine Shop / https://neurophysics.ucsd.edu/lab_hardware.php | Rev 01 2011 Part A |
| Patch Clamp Amplifier | Molecular Devices | Cat # Multiclamp 700B |
| Confocal microscope | Leica | TCS SP8 STED 3X |
| Multiphoton microscope | Olympus | FVMPE-RS |
| Single Unit Recording Amp | TDT | RZ5 |
| Digitizer and Sequencer | CED | Micro 1401 |
| LED for slice illumination | Mightex | LCS-0470–50-22 |
| Miltenyi Tyto microfluidic sorter | Miltenyi Biotec | Cat #130–103-931 |