FIG. 4.
Effect of algC complementation on the localization of β-lactamase (Bla) and the production of elastase in strain PAO1algC. LasB and β-lactamase were immunodetected by chemiluminescence as indicated in Materials and Methods. Samples were withdrawn at OD600s of 3.9, 3.7, and 3.6, respectively, for the PAO1(pUCP18), PAO1algC(pUCP18), and PAO1algC(pLPS188) strains. Only the relevant parts of the immunoblots are shown. C, cellular fraction; E, extracellular fraction. The positions of LasB (33 kDa) and β-lactamase (30 kDa) are indicated by arrows.