FIG. 5.
Effect of LPS alteration on the organization of the Xcp machinery (A) and on the multimerization of XcpQ (B). (A) Cellular proteins from the PAO1 and PAO1algC strains were analyzed by Western blotting and immunodetection of XcpR (55 kDa), XcpT (15 kDa), XcpY (41 kDa), and XcpQ (70 kDa) carried out by chemiluminescence with their respective antisera. (B) Cellular proteins from the PAO1 and PAO1algC strains were solubilized either at 20°C (−) or at 95°C (+) and analyzed by Western blotting. Immunodetection was performed with XcpQ-directed antiserum, and proteins were detected by chemiluminescence. Only the relevant parts of the immunoblots are shown. Samples were withdrawn at OD600s of 2.2 (strain PAO1) and 2.1 (strain PAO1algC).