TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| P. aeruginosa | ||
| PAO1 | Prototroph, chl-2 | |
| AK1012 | Rough LPS derivative of PAO1, core deficient, A− B− | 21 |
| PAO1algC | algC mutant derivative of PAO1 obtained by Tc cartridge insertion, A− B− | 8 |
| PAO1/::xcpR′ | Obtained by insertion of pGMR′ suicide vector in xcpR | This study |
| PAO1algC/::xcpR′ | idem as PAO1/::xcpR′ | This study |
| E. coli TG1 | supE hsd Δ thi Δ(lac-proAB) F′ (traD36 proAB+ lacIqlacZΔM15) | |
| Plasmids | ||
| pAX24 | Tcr, IncP1 oriT pLAFR3 with xcpP to −Z on 20-kb DNA insert | 13 |
| pUC19 | Apr, ColE1, lacI, φ80dlacZ | 48 |
| pUCR | xcpR gene cloned into the SmaI site of pUC19 | This study |
| pMMB67EH | Apr, RSF 1010 (IncQ), tac promoter | 15 |
| pUCBM20 | Apr, ColE1 | Boehringer |
| pGMR′ | Apr, 0.5-kb SalI internal fragment of xcpR from pUCR cloned into the SalI site of pUCBM20 | This study |
| pUCP18 | Apr, ColE1, broad-host-range plasmid | 44 |
| pML27 | Apr, lasB in pMMB67EH | 11 |
| pLPS 188 | Apr, algC gene in pUCP18 | 17 |
| pJRD215 | Kmr, RSF 1010, wide-host-range cosmid-cloning plasmid | 9 |
| p24 | Kmr, P. alcaligenes lipAB genes cloned into pJRD215 | Similar to pJRDlipAB (16) |
| pTS400 | Apr, translational lasB-lacZ fusion in pSW205 | 37 |