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. 2022 Aug 24;2022:1911592. doi: 10.1155/2022/1911592

Figure 5.

Figure 5

PAK1 is a direct target of miR-485-5p. (a) The binding site between miR-485-5p and the 3′-UTR of PAK1. (b) The PAK1 expression in PC cell lines was determined by the qRT-PCR assay. (c) The luciferase reporter assay was conducted to investigate the connection between miR-485-5p and PAK1. (d) Western blot analysis was used to examine the PAK1 expression. (e) The success of PAK1 inhibition with sh-PAK1 was confirmed by qRT-PCR analysis. (f) The CCK-8 assay was used to determine cell proliferation. (g–i) Wound healing and transwell assays were carried out to detect cell invasion and migration. (j) The TUNEL assay was used to determine cell apoptosis. ∗∗P < 0.01; ∗∗∗P < 0.001.