Table 2.
Week 10 change from baseline T cell populations of dogs consuming a SCFP-supplemented or control diet
| Item | Δ Control | Δ SCFP | SEM | P-value |
|---|---|---|---|---|
| Unstimulated | ||||
| Lymphocyte, % of PBMC1 | 8.30a | –3.89b | 4.19 | 0.0440 |
| T cell, % of lymphocyte | –8.17 | –3.20 | 3.12 | 0.2721 |
| Helper T cell, % of lymphocyte | 3.93 | 3.17 | 2.02 | 0.7911 |
| Cytotoxic T cell, % of lymphocyte | –15.61 | –20.40 | 5.42 | 0.2858 |
| Helper: cytotoxic T cell ratio | 10.48 | 13.96 | 9.54 | 0.7993 |
| IFN-γ secreting T cell, % of lymphocyte | 3.75 | 0.88 | 1.50 | 0.3568 |
| IFN-γ secreting helper T cell, % of lymphocyte | 0.00 | 0.01 | 0.01 | 0.2220 |
| IFN-γ secreting cytotoxic T cell, % of lymphocyte | 12.82 | 14.32 | 4.56 | 0.8174 |
| Stimulated2 | ||||
| Lymphocyte, % of PBMC1 | –1.75 | –6.28 | 2.05 | 0.1301 |
| T cell, % of lymphocyte | –12.29b | –4.01a | 3.23 | 0.0350 |
| Helper T cell, % of lymphocyte | 4.88 | 0.56 | 2.28 | 0.1388 |
| Cytotoxic T cell, % of lymphocyte | -6.32 | -21.13 | 5.68 | 0.0759 |
| Helper: cytotoxic T cell ratio | 2.15 | 13.32 | 4.30 | 0.1989 |
| IFN-γ secreting T cell, % of lymphocyte | 2.18a | –10.94b | 4.09 | 0.0309 |
| IFN-γ secreting helper T cell, % of lymphocyte | –1.53 | –3.76 | 2.27 | 0.4916 |
| IFN-γ secreting cytotoxic T cell, % of lymphocyte | 8.31 | 10.87 | 4.46 | 0.6876 |
PBMC = peripheral blood mononuclear cells.
Cells were stimulated with cell stimulation cocktail (phorbol 12-myristate 13-acetate, ionomycin, brefeldin A, and monensin) for 4 h.