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. 2022 Aug 24;609(7925):191–196. doi: 10.1038/s41586-022-05114-0

Fig. 3. Target pairing past the seed region undocks the HNH nuclease domain.

Fig. 3

a, Position of the HNH catalytic site in the binary, 6-, 8- and 10-nt match complexes. b, Structural overlay of the REC2 and REC3 domains in the 10-, 12-, 14-, 16- and 18-nt match (checkpoint) complexes. c, Overview of the 12-nt match (left), 14-nt match (middle) and 16-nt match (right) complexes, shown in the same orientations. For each complex, the unsharpened cryo-EM map is overlaid with the respective atomic model. The 12-nt match complex map shows residual density for the displaced NTS (white). The 14-nt match map reveals residual density corresponding to the HNH domain. No density is visible for NTS. Cryo-EM maps are coloured according the schematic in Fig. 1a.