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. 2022 Aug 24;609(7925):191–196. doi: 10.1038/s41586-022-05114-0

Fig. 4. HNH domain rotation and DNA bending enable catalytic activation.

Fig. 4

a, The structure of the 18-nt match complex in the pre-cleavage, checkpoint state. b, The structure of the 18-nt match complex in the catalytically active state. c, Conformations of the guide–target heteroduplexes and REC2 and REC3 domains in the 18-nt match checkpoint (left) and catalytic (right) complexes. The structures are shown in the same orientations as in a,b. The HNH domain has been omitted from the images for clarity. d, Zoomed-in view of the HNH nuclease active site in the 18-match catalytic complex containing bound cleaved TS. e, Zoomed-in view of the L1 linker contacting the minor groove of the guide RNA–target DNA heteroduplex. f, Zoomed-in view of the RuvC nuclease active site containing the 3′-terminal product of cleaved NTS.