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. 2022 Aug 24;609(7925):191–196. doi: 10.1038/s41586-022-05114-0

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© The Author(s) 2022, corrected publication 2023

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Extended Data Fig. 1 — Size exclusion chromatography analysis of nuclease-inactive SpCas9 complexed with sgRNA and Cy5-labeled DNA substrates with increasing extent of guide-target complementarity. A254/A280 and Cy5 signals were normalised.