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. 2022 Aug 24;609(7925):191–196. doi: 10.1038/s41586-022-05114-0

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© The Author(s) 2022, corrected publication 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 5 — a, Structural overlays of the SpCas9 bridge helix (BH), REC1, RuvC, and PAM-interacting (PI) domains, as well as the PAM-proximal DNA duplex and the sgRNA from the partially bound complex structures determined by crystallography and cryoEM, and full R-loop complexes (PDB: 6O0X, 6O0Y, 6O0Z)²⁸. b, Zoom-in view of the PAM-distal DNA duplex in the 6-nt match complex. The protein surface is coloured according to electrostatic surface potential, with red denoting negative and blue positive charge. c, Interactions between SpCas9 REC2 domain and the backbone of the PAM-distal NTS in the 6-nt match complex. d, Interactions between the REC3 domain and the backbone of the PAM-distal TS in the 6-nt match complex. e, Central slice through the 6-nt match complex. Cryo-EM density map is coloured according to Fig. 1a. White density indicates positioning of the 5’ sgRNA end. f, PAM-distal DNA duplex in the 8-nt match complex remains positioned in a positively charged cavity between the REC2 and REC3 domains. The protein surface is coloured according to electrostatic surface potential. g, Interactions of the REC2 domain with the PAM-distal DNA duplex in the 8-nt match complex. h, Interactions of the REC3 domain with the NTS of the PAM-distal duplex in the 8-nt match complex.