Fig. 1.

Collection and extraction of SARS-CoV-2 virus via passive sampling. (A) Torpedo sampler pictured empty (left side), with electronegative filter membrane and cotton gauze (middle), and assembled (right side); (B) demonstration of manhole torpedo sampler deployment setup showing sampler connected to manhole cover using high tensile wire; (C) flowchart describing the procedural steps for measuring SARS-CoV-2; (D) raw wastewater samples mixed with elution buffer in 15 mL centrifuge tubes; (E) Benchmark Block™ Mini Dry Bath incubator used for RNA concentration and extraction; and (F) magnetic racks with 2 mL samples tubes containing magnetic beads and eluted RNA sample.