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. 2022 Jun 23;27(2):145–153. doi: 10.1007/s10911-022-09521-x

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — SHP2 blockade downregulates IL-8. (A) Bar graph representing the quantification of the nine most downregulated cytokines in SUM159 cell supernatants treated with SHP099 for 48 h relative to vehicle-treated cells. Data shown are dot quantification by pixel density from cytokine-array scans. (B) Bar graph representing IL-8 protein abundance in the supernatant of the indicated cell lines treated for 48 h at the SHP099 concentrations shown. Data are IL-8 protein concentrations normalized by total protein ± standard deviation (S.D.) (n = 3, ****P ≤ 0.0001, Two-way ANOVA test). (C) Bar graph representing CXCL8 mRNA expression in the indicated cell lines after 120 h of SHP099 inhibition (n = 3, **P ≤ 0.001, Two-way ANOVA test). (D) Bar graph representing IL-8 protein abundance in the supernatant of the indicated cell lines treated for 24 h with DMSO or 10 µM SHP099 normalized to the protein content of each well determined by SRB staining. Washout conditions were collected 24 h after media exchange. (n = 3, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, Two-way ANOVA test). (E) Bar graph representing IL-8 protein abundance in the supernatants of the indicated cell lines upon SHP2 knockdown. Data are shown ± S.D. (n = 3, ****P ≤ 0.0001, Two-way ANOVA test). (F) Bar graph representing CXCL8 mRNA expression in the indicated cell lines upon SHP2 knockdown. Data are shown ± S.D. (n = 3, *P ≤ 0.05, **P ≤ 0.01; Two-way ANOVA test)