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. 2022 Mar 16;29(9):1864–1873. doi: 10.1038/s41418-022-00971-8

Fig. 6. MYC contributes to OTUB1 mediated breast tumorigenesis in vitro and in vivo.

Fig. 6

A MCF-7 or MB231 cells with OTUB1 depletion reconstituted stable overexpression of MYC. Total cell lysates were prepared, and immunoblotting analyse was performed. B MCF-7 or MB231 cells with OTUB1 depletion reconstituted stable overexpression of MYC. CCK-8 assays were performed. C MCF-7 or MB231 cells with OTUB1 depletion reconstituted stable overexpression of MYC. Clone formation assays were performed. D MCF-7 or MB231 cells with OTUB1 depletion reconstituted stable overexpression of MYC. Wound healing assays were performed (Scale bar, 50 μm). E MCF-7 or MB231 cells with OTUB1 depletion reconstituted stable overexpression of MYC. Cell invasion assays were performed (Scale bar, 50 μm). F–H MCF-7 cells with OTUB1 depletion reconstituted stable overexpression of MYC were subcutaneously injected into nude mice. After 3 weeks, the mice were sacrificed and dissected at the endpoint. Tumor growth and weight were examined. I Representative images of H/E staining and Ki67 staining of tumor samples (Scale bar, 20 μm). (All data represent mean ± SEM n ≥ 3), *p < 0.05.