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. 2022 Feb 25;29(9):1744–1756. doi: 10.1038/s41418-022-00959-4

Fig. 3. CRABP1 is highly and specifically expressed in spinal MNs.

Fig. 3

A, B Western blot analyses of various tissues and brain regions from 2-months old WT mice for the expression of CRABP1, NeuN. β-actin was used as a loading control. C Immunostaining of a spinal cord section (left) and bright field image (right) from 2-months-old WT mouse. CRABP1 is detected in the ventral horn, a region where motor neurons also localize. D Immunostaining of spinal cord section with CRABP1 (red), ChAT (motor neuron marker; green, upper panel), and GFAP (glial cell marker; green; bottom panel). E Immunostaining of lumbrical muscle from 2 months old mice to monitor pre-synaptic axon terminal marker Tuj1 (red; upper left), AChR cluster marker (αBTX; green upper right panel), and CRABP1 (White; bottom left). Overlay of Tuj1, αBTX, and CRABP1 images is shown in lower right.